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KMID : 0380020200350030266
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Korean Journal of Biotechnology and Bioengineering
2020 Volume.35 No. 3 p.266 ~ p.272
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Chondrogenic Potential of Canine Chondrocytes through Electrical Stimulation
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Gu Na-Yeon
Lee Se-A
Jeong So-Yeon
Lee Jienny
Lee Yoon-Hee
Hyun Bang-Hun
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Abstract
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Electrical stimulation (ES) is known to guide the development and regeneration of many tissues. Cartilage has no direct blood supply, thus it has little or no capacity to repair itself. Articular cartilage is to provide a smooth lubricating surface to the joints and facilitates the transfer of loads with a low frictional coefficient. Meniscus is cartilaginous tissues that provide structural integrity to the knee when it undergoes tension and torsion. We tried to isolate and characterize chondrocytes from articular cartilage (Ar-chon) and meniscus (Me-chon) of dogs. Also, we exposed Me-chon to ES and assessed changes in the chondrogenic potential. As results, Ar-chon and Me-chon had fibroblasts-like morphology. The proliferative capacity of Me-chon was higher than those of Ar-chon in the early passage. Both cells expressed cell surface markers such as CD29, CD44, and CD90, but did not express CD34, CD117, and MHC-2. ES was applied to Me-chon cultured under condition of high-density micromass under ES of 10 V/cm, with duration of 10 ms and a frequency of 2 Hz for three days. And then, gene expression of chondrogenic markers such as type II collage alpha 1, Aggrecan, and SOX9 was analyzed. As a result, we observed the upregulated expression level of chondrogenic markers of Me-chon by specific ES in absence of exogenous factors. Also, we found that ES significantly upregulated expression level of heat shock protein, which affects cartilage formation. Taken together, ES-driven Me-chon is thought to be useful for basic and therapeutic research applications in the early passage.
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KEYWORD
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canine, chondrocytes, chondrogenic potential, electrical stimulation
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